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endogenous alas1  (MedChemExpress)
94
MedChemExpress endogenous alas1
( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state <t>ALAS1</t> levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).
Endogenous Alas1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alas1+antibody/ALAS1+Antibody/pmc13425942-201-4-16
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endogenous alas1 - by Bioz Stars, 2026-09
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alas1 polyclonal antibody  (Thermo Fisher)
90
Thermo Fisher alas1 polyclonal antibody
( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state <t>ALAS1</t> levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).
Alas1 Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alas1+antibody/alas1+polyclonal+antibody/pmc09585024__41467_2022_33837_MOESM3_ESM-29-0-3
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alas1 polyclonal antibody - by Bioz Stars, 2026-09
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alas1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology alas1
( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state <t>ALAS1</t> levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).
Alas1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alas1+antibody/ALAS-H+Antibody/pm41691085-226-20-21
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1 ap  (Proteintech)
92
Proteintech 1 ap
( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state <t>ALAS1</t> levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).
1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit  (Proteintech)
92
Proteintech rabbit
( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state <t>ALAS1</t> levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).
Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alas1+antibody/ALAS1+Polyclonal+antibody/pmc11181659-3-2-4
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alas1  (Proteintech)
92
Proteintech alas1
( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state <t>ALAS1</t> levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).
Alas1, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alas1+antibody/ALAS1+Polyclonal+antibody/pmc10981497-418-35-66
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Image Search Results


( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state ALAS1 levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).

Journal: Science (New York, N.Y.)

Article Title: An adaptor for feedback regulation of heme biosynthesis by a mitochondrial protease

doi: 10.1126/science.ads5397

Figure Lengend Snippet: ( A ) In vitro degradation of ALAS2 by CLPXP with ATP, +/−POLDIP2 (PD2) and hemin (H) as indicated. SDS-PAGE gels of samples were stained with Sypro Red. Upper band (arrow) is full-length ALAS2; lower band (asterisk) corresponds to C-terminally proteolyzed ALAS2 fragment ( 20 ). fig. S1A displays full gel images. ( B ) Plot of degradation of ALAS2 as monitored in (A). Lines represent linear fits to data. N = 5 for +hemin +PD2, 3 for other conditions; error = SEM. ( C ) Rates extracted from (B) with error of fit to all replicates. CLPX 6 refers to a CLPX hexamer. ( D ) Protein immunoblot of steady-state ALAS1 levels in wt or PD2-KO HEK293 cells (three independent cultures each). ( E ) Degradation of ALAS1 in wt or PD2-KO HEK293 cells. Heme production was blocked with 0.5 mM succinylacetone starting 24 hours before observation. At t 0 , 100 μg/mL cycloheximide and 100 μM hemin or vehicle (DMSO) was added. Proteins were detected by Western blot. fig. S1C displays full blot images. ( F ) Quantitation of ALAS1 from (E) (n = 3, error = SEM, p = 0.01 for more ALAS1 remaining after 3h in PD2-KO compared to wt cells).

Article Snippet: To monitor degradation of endogenous ALAS1, cells were plated 72 hours prior to observation and succinylacetone (MedChemExpress HY-W010184) was added to 0.5 mM 24 hours prior to observation.

Techniques: In Vitro, SDS Page, Staining, Western Blot, Quantitation Assay